Loop-linker PCR: An advanced PCR technique for genome walking
نویسندگان
چکیده
منابع مشابه
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A chromosome walking method was improved in this work. The new method was named as nonspecific primer anchored PCR (NPA-PCR). Nested gene specific primers were designed based on the known region and long random primer using degeneracy oligonucleotides for nonspecific anchoring. Annealing temperatures were varied to control the priming. Target sequences were obtained by PCR with random primer an...
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ژورنال
عنوان ژورنال: IUBMB Life
سال: 2012
ISSN: 1521-6543
DOI: 10.1002/iub.1069